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Professional Tramadol Test Kit  Rapid Diagnostic Card Format 24 Month Shelf Life

Categories Drug Abuse Test Kit
Brand Name: New Life
Model Number: cassette
Certification: ISO13485, CE
Place of Origin: China
MOQ: 5000pcs
Price: USD0.5-USD5
Payment Terms: T/T, Western Union, MoneyGram
Supply Ability: 2000000pcs/month
Delivery Time: 20-30days
Packaging Details: 1pc/pouch, 25pcs/box
Category: Drug TESGTING kits
Format: card
Specimen: Urine
Testing time: 5-15 minutes
Shelf Life: 24 Months
application: Tramadol
cut-off: 100ng/ml
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    Professional Tramadol Test Kit  Rapid Diagnostic Card Format 24 Month Shelf Life

    New Drug Abuse Test Kit TML (Tramadol) Rapid Diagnostic Test dipcard, cut-off 100ng/ml urine drug test, professional use

    Product Name

    One Step TML (Tramadol) Rapid Diagnostic Test dipcard

    Specimen: Urine

    ProductCat NoSpecimenSensitivityFormatKit Size
    TMLYD171S3Urine100 ng/mL3mm Strip100T
    YD171C44mm Cassette25T

    Intended Use

    The TML Rapid Test Cassette (Urine) is a rapid immunochromatographic assay for the qualitative detection of Tramadol in human urine at a cut-off concentration of 100 ng/mL. This assay provides only a preliminary analytical test result. A more specific alternate chemical method must be used in order to obtain a confirmed analytical result. Gas chromatography/mass spectrometry (GC/MS) is the preferred confirmatory method. Clinical consideration and professional judgment should be applied to any drug of abuse test result, particularly when preliminary positive results are used.

    ParameterCalibratorCut-off (ng/mL)

    Test Principle

    The TML Rapid Test Cassette (Urine) is a a rapid chromatographic immunoassay based on the principle of competitive binding. Drugs which may be present in the urine specimen compete against the drug conjugate for binding sites on the antibody. During testing, a urine specimen migrates upward by capillary action. Tramadol, if present in the urine specimen below 100 ng/mL, will not saturate the binding sites of antibody-coated particles in the test device. The antibody-coated particles will then be captured by immobilized Tramadol conjugate and a visible colored line will show up in the test line region. The colored line will not form in the test line region if the Tramadol level exceeds 100 ng/mL because it will saturate all the binding sites of anti-Tramadol antibodies. A drug-positive urine specimen will not generate a colored line in the test line region because of drug competition, while a drug-negative urine specimen or a specimen containing a drug concentration less than the cut-off will generate a line in the test line region. To serve as a procedural control, a colored line will always appear at the control line region, indicating that proper volume of specimen has been added and membrane wicking has occurred


    1. Bring tests, specimens, and/or controls to room temperature (15-30°C) before use.

    Remove the test from its sealed pouch, and place it on a clean, level surface. Label the test with patient or control identification. For best results, the assay should be performed within one hour.

    2. Using the provided disposable pipette, transfer 3 drops of specimen (approximately 120 µL) to the specimen well (S) of the device and start the timer.

    3. Avoid trapping air bubbles in the specimen well (S), and do not add any solution to the result area.

    4. As the test begins to work, color will migrate across the membrane.

    5. Wait for the colored band(s) to appear. The result should be read at 5 minutes. Do not interpret the result after 8 minutes


    NEGATIVE:* A colored line appears in the Control region (C) and colored lines appears in the Test region (T). This negative result means that the concentrations in the urine sample are below the designated cut-off levels for a particular drug tested.

    *NOTE: The shade of the colored lines(s) in the Test region (T) may vary. The result should be considered negative whenever there is even a faint line.

    POSITIVE: A colored line appears in the Control region (C) and NO line appears in the Test region (T). The positive result means that the drug concentration in the urine sample is greater than the designated cut-off for a specific drug.

    INVALID: No line appears in the Control region (C). Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for Control line failure. Read the directions again and repeat the test with a new test card. If the result is still invalid, contact your manufacturer.


    A procedural control is included in the test. A line appearing in the control region

    (C) is considered an internal procedural control. It confirms sufficient specimen volume, adequate membrane wicking and correct procedural technique.Control standards are not supplied with this kit. However, it is recommended that positive and negative controls be tested as good laboratory practice to confirm the test procedure and to verify proper test performance.

    Contact:Jerry Meng
    Email:Jerry @ newlifebiotest .com
    Tel.+86 18657312116

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