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Drug Abuse Test Kit BAR (Barbiturates) Rapid Diagnostic Test strip , urine drug test, cut-off 300ng/ml

Categories Drug Abuse Test Kit
Brand Name: New Life
Model Number: strip
Certification: ISO13485, CE
Place of Origin: China
MOQ: 5000pcs
Price: USD0.5-USD5
Payment Terms: T/T, Western Union, MoneyGram
Supply Ability: 2000000pcs/month
Delivery Time: 20-30days
Packaging Details: 1pc/pouch, 25pcs/box
Category: Drug test kit
Format: Strip
Specimen: Urine
Testing time: 5-15 minutes
Shelf Life: 24 Months
application: BAR
cut-off: 300ng/ml
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    Drug Abuse Test Kit BAR (Barbiturates) Rapid Diagnostic Test strip , urine drug test, cut-off 300ng/ml

    Drug Abuse Test Kit BAR (Barbiturates) Rapid Diagnostic Test strip , urine drug test, cut-off 300ng/ml

    Product Name

    BAR Rapid Diagnostic Test/ Barbiturates Rapid Diagnostic Test
    Specimen: Urine

    Intended Use

    Barbiturates are central nervous system depressants. They are used therapeutically as sedatives, hypnotics, and anticonvulsants. Barbiturates are almost always taken orally as capsules or tablets. The effects resemble those of intoxication with alcohol. Chronic use of Barbiturates leads to tolerance and physical dependence. Short acting Barbiturates taken at 400 mg/day for 2-3 months produce a clinically significant degree of physical dependence. Withdrawal symptoms experienced during periods of drug abstinence can be severe enough to cause death. Only a small amount (less than 5%) of most Barbiturates are excreted unaltered in the urine. The detection period for the Barbiturates in the urine is 4-7 days.1
    The BAR One Step Barbiturates Test Strip (Urine) is a rapid urine screening test that can be performed without the use of an instrument. The test utilizes a monoclonal antibody to selectively detect elevated levels of Barbituratess in urine. The BAR One Step Barbiturates Test Strip (Urine) yields a positive result when Barbituratess in urine exceed 300 ng/mL

    BAR (Barbiturates)Secobarbital300

    Test Principle

    The BAR One Step Barbiturates Test Strip (Urine) is a rapid chromatographic immunoassay based on the principle of competitive binding. Drugs which may be present in the urine specimen compete against the drug conjugate for binding sites on the antibody.
    During testing, a urine specimen migrates upward by capillary action. Barbiturates, if present in the urine specimen below 300 ng/mL, will not saturate the binding sites of antibody-coated particles in the test strip. The antibody-coated particles will then be captured by immobilized Barbiturates conjugate and a visible colored line will show up in the test line region. The colored line will not form in the test line region if the Barbiturates level exceeds 300 ng/mL because it will saturate all the binding sites of anti-Barbiturates antibodies.
    A drug-positive urine specimen will not generate a colored line in the test line region, while a drug-negative urine specimen or a specimen containing a drug in a concentration less than the cut-off will generate a colored line in the test line region. To serve as a procedural control, a colored line will always appear at the control line region, indicating that proper volume of specimen has been added and membrane wicking has occurred.


    Bring tests, specimens, and/or controls to room temperature (15-30°C) before use.
    Remove the test from its sealed pouch, and place it on a clean, level surface. Label the test with patient or control identification. For best results, the assay should be performed within one hour.

    Using the provided disposable pipette, transfer 3 drops of specimen (approximately 120 µL) to the specimen well (S) of the device and start the timer.

    Avoid trapping air bubbles in the specimen well (S), and do not add any solution to the result area.
    As the test begins to work, color will migrate across the membrane.

    Wait for the colored band(s) to appear. The result should be read at 5 minutes. Do not interpret the result after 8 minutes


    NEGATIVE:* A colored line appears in the Control region (C) and colored lines appears in the Test region (T). This negative result means that the concentrations in the urine sample are below the designated cut-off levels for a particular drug tested.

    *NOTE: The shade of the colored lines(s) in the Test region (T) may vary. The result should be considered negative whenever there is even a faint line.

    POSITIVE: A colored line appears in the Control region (C) and NO line appears in the Test region (T). The positive result means that the drug concentration in the urine sample is greater than the designated cut-off for a specific drug.

    INVALID: No line appears in the Control region (C). Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for Control line failure. Read the directions again and repeat the test with a new test card. If the result is still invalid, contact your manufacturer.


    A procedural control is included in the test. A line appearing in the control region
    (C) is considered an internal procedural control. It confirms sufficient specimen volume, adequate membrane wicking and correct procedural technique.
    Control standards are not supplied with this kit. However, it is recommended that positive and negative controls be tested as good laboratory practice to confirm the test procedure and to verify proper test performance.

    performance characteristics

    Analytical Sensitivity

    A drug-free urine pool was spiked with Barbiturates at the following concentrations: 0 ng/mL, 150 ng/mL, 225 ng/mL, 300 ng/mL, 375 ng/mL, 450 ng/mL and 600ng/mL. The result demonstrates > 99% accuracy at 50% above and 50% below the cut-off concentration. Results are presented in Table 2 below.

    Table 2: Analytical Sensitivity Summary

    BAR Concentration (ng/mL)Percent of Cut-offnVisual Result

    Conclusion: As indicated in table above: all specimens with BAR concentration equal to or lower than 150ng/mL show negative results, all specimens with BAR concentration of 300ng/mL are identified as “+/-”, and all specimens with BAR concentration equal to or higher than 450ng/mL showed positive results. Therefore, the cut-off concentration of the BAR One Step Test Strip (Urine) is determined to be 300ng/mL BAR.

    Analytical Specificity

    Table 3 lists the compounds that are positively detected in urine by the BAR One Step Barbiturates Test Strip (Urine) at 5 minutes and the concentrations at which they are detected.

    Table 3: Analytical Specificity

    CompoundConcentration (ng/mL)CompoundConcentration (ng/mL)

    Contact:Jerry Meng
    Email:Jerry @ newlifebiotest .com
    Tel.+86 18657312116

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