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| Categories | Covid Test Kit |
|---|---|
| Brand Name: | Ascentet |
| Model Number: | Covid Test Kit |
| Certification: | CE FDA TGA |
| Place of Origin: | China |
| MOQ: | 10000pcs |
| Price: | $1.50-2.0/pcs |
| Payment Terms: | T/T, Western Union, MoneyGram |
| Supply Ability: | 100000pcs/day |
| Delivery Time: | 5-20 days |
| Packaging Details: | 25tests/box 1test/box |
| Sample Collection:: | Nasal Swab |
| Package: | 25pcs/box, 1pcs/box |
| Use: | Fast Check Of Coronavirus |
| Reading Time: | In 15 Mins |
| Qualitative: | Qualitative Determination Of Covid-19 |
| Method: | Colloidal Gold Assay |
| Company Info. |
| Ascentet Group Co.,Ltd |
| View Contact Details |
| Product List |
Introduction
Covid-19 first emerged in December 2019 in Wuhan, China. The cause
of the disease was confirmed as a new kind of coronavirus, this
virus is transmitted through air from people to people. In two
months, the infection has quickly spread in China and to a number
of countries around the world.
Covid-19 virus causes over 2% mortality, there is 7 to 14 days
incubation period. The current diagnostic method is majorly by PCR
test, but its overall sensitivity is 50 to 60% and is limited by
the user with the special trainning and special instruments. There
is an urgent need to develop a simple and fast test to screen large
population and unsymptomatic infected people. Infectious disease
usually produces specific IgM antibody at 2 to 4 days after the
infection, while specific IgG antibody appears after 5 to 7 days.
Compared to other test, this test is more convenient with low cost,
it can be performed in field, mobile hospital and laboratory.
Safety measure:
Covid-19 antibody test must be manipulated in a biosafety Level 3
facility, or with good personal protection, specimen deposit have
to follow WHO or local health authority guidline .
Assay principle
The recombinant Covid-19 nuceloprotein antigen is used in the test,
serum, plasma or whole blood from Covid-19 infected patient is
loaded to the device, the specific Covid-19 antibodies in specimen
will bind to gold-conjugated Covid-19 nucleoprotein antigens
forming antibody – colloid gold-antigen complex. While the complex
migrates along nitrocellulose membrane, the complex is captured on
the test lines coated with anti-human IgM or IgG causing a pink to
deep pink band at the IgG or IgM test line. The signal intensity is
variable depending on the amount of specific antibody present in
specimen. A red control line should always develop over control
line indicating the proper performance.
Prior to testing, all reagents were returned to room
temperature.Testing shall be performed at room temperature.
I. Sample extraction (see Figure 1)
1. Add 400 L (about 10 drops) of sample buffer vertically to the
sample extraction tube and then insert the sample into the tube and
rotate against the inner wall about 10 times so that the samples in
the solution dissolve as much as possible.
2. Squsample q-tip along the inner wall of the extraction tube to
retain as much liquid in the tube as possible from the sample, then
removed and discarded.
3. Cover the dropper.
two.Test procedure (see Figure 2)
1. Remove the test card from the sealing bag.
2. Add 2 drops (about 80 L) of the treated sample extract to the
sample hole of the test card and start the timer.
3. The results were read after placing the test card for 15 minutes
at room temperature.The results were invalid after 20 minutes.
Schematic diagram of the test card result judgment:
Invalid ① result: the quality control line (C line) has no reaction
line, the detection is invalid, and the test shall be repeated.
② negative result: red ribbon, quality control line (C line) is in
color.
③ positive result: two red bands, detection line (T line) and
quality control line (C line) are in color.
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